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GH3, MMQ, AtT20, and primary AP cells were plated in growth medium in six well plates at 1 10 5 cells per well 1 day before infection in the presence or absence of the tetracycline analog doxycycline 500 ng ml
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Infections occurring in men, pregnant women, patients with abnormal urologic anatomy, indwelling catheters, or frequent instrumentation also are defined as being complicated